RPRD | Comparison of Radiochemical yield of FDG using HPLC and TLC

Yong-Hyun Cho, Hong-Jae Lee, Yong-Seok Lee, Jae-Hyung Lee, Hyeong-Jin Kwon, Hyun-Ju Kim

PET center, Department of Nuclear Medicine, Seoul National University Hospital, Seoul, Korea

Purpose

TLC(Thin Layer Chromatography) have been used to confirm the radiochemical yield after synthesizing [18F]FDG. But TLC scanner dose not let us know other information such as chemical impurity or quantity of compound which did not have a radioactive decay. Many scientists reported that they used HPLC system for Q.C of [18F]FDG(Ryuji Nakao,  et  al.  Nuclear Medicine  and  Biology  32(2005)  907-  912).  So,  we  tried  to compare two methods and which one was better.

Materials and Methods

We used AR2000 TLC Scanner(BIOSCAN co., USA), 321 pump, UV/VIS-155 detector(GILSON co., FRANCE) and Gabi RI detector(RAYTEST co., GERMANY). [18F]FDG   was   produced   using   SNUH   Cyclotron   facilities   (TR13,   EBCO   co., CANADA). We purchased analytical column (Inertsil NH2., GL science, JAPAN) and FDG standard (ABX co., GERMANY).

Conclusion

Using TLC scanner, we got the results 15 min later. The results showed us several radio- compound  peaks  such  as  free [18F],  [18F]FDG,  [18F]TAG,  and  partially  hydrolyzed [18F]TAG. In case of HPLC, we got the results by 18 min. The results showed us not only above all radio peaks but also UV peaks such as FDG, ClDG and other unidentified compound. But there was a pre-preparation to execute a HPLC method.

Key words: [18F]FDG, ClDG, Q.C, radiochemical yield, chemical impurity